Composite
CircNpu*

Part:BBa_K1362001

Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha   Group: iGEM14_Heidelberg   (2014-10-07)

NpuDnaE intein RFC[105] circularization construct (with FLAG and Smt3)

For detailed information see BBa_K1362000. Addition of BBa_K1362428 and BBa_K1362429 might improve expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 160
    Illegal BamHI site found at 351
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1249
    Illegal AgeI site found at 1361
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1538
    Illegal BsaI.rc site found at 463


[edit]
Categories
Parameters
None